Bioremediation Process
BioTherm Eliminator exhibits an exclusive biodegradation stimulation capability. Extensive lab and field testing indicates that it acts like a synthetic enzyme catalyst, without the limitations of temperature and pH associated with biological enzymes1. BioTherm quickly catalyzes the degradation of alkanes, cellulose wates, hydrocarbons, oils, and sewage without itself being depleted and is under Canadian Government approval as environmentally safe.
BioTherm Eliminator Benefits:
- In situ bioremediation of contaminated water and soils.
- Eliminates Phosphorus and Nitrates from the wastes by utilising them as a source of energy in stimulating bacteria to increased aerobic respiration (P went from 306 ppm to 2 ppm in lab. test).
- Rapid biodegradation of hydrocarbons and organic wastes such as sewage, manure, and cellulose wastes from agricultural and food processing.
- Eliminates Struvite in sewage by dispersion of the calcium, magnesium, and uric acid deposits.
- Promotes aerobic biodegradation of wastes and eliminates wastes stench.
Bioremediation Treatment Process with BioTherm Eliminator (the Waste Digester)
Sulphates and organic compounds are fully biodegradable utilising BioTherm Eliminator. Field testing consistently shows immediate biodegradation of hydrocarbons, confirming this with a proven 5% hydrocarbon biodegradation ratio in solution with a 93% water base and a 2% BioTherm base. Tests were run using GreenCut Cutting Fluid.
BioTherm Eliminator, GreenCut Cutting Fluid, and Lubritherm Hydraulic Fluid are quite similar in their chemical construction in that they share the ability to biodegrade hydrocarbons, cellulose, and sewage; besides their unique and very effective abilities to operate within their individual specialties.
Further unofficial tests with about 30% oil, mixed with 68% water and 2% GreenCut have been run in small shop CNC equipment for many years, where they may have severe seal and way oil leakage (tramp oils) on some older metal machining equipment.
Lubritherm Hydraulic Fluid has proven in "field testing" that it biodegrades up to 30% of hydraulic oil by volume -over an estimated time period of about 4 weeks, which was added inadvertently by the Client in Manitoba about 6 years ago. Also -the oil heaters in the same 90 US Gals. hydraulic reservoir were left on for 4 to 6 months, causing extreme operational Lubritherm fluid temperatures to 140°C. This hydraulic unit is still operating today on the original Lubritherm Hydraulic Fluid with complete satisfaction.
Unofficial tests with 1% GreenCut in large CNC plasma arc tanks of some 15,000 US Gals. capacity, have consistently claimed complete biodegradation of oils and other organic compounds over the last four years where large quantities of organic compounds covered steel plates move through the bath on a 16 hr. x 6 d. week basis. In this instance the fluid has not been changed over the last 4 years, just more added, with excellent results, showing clean baths with no smells, i.e. no anaerobic bacteria formation whatsoever. Normally, GreenCut does not need to be changed out for fresh mixtures. Fresh premix of GreenCut/water is added to the tank as the tank GreenCut coolant package is used up.
BioTherm Eliminator enables the conversion to carboxylic acids that biodegrade to a harmless reduction of carbon dioxide, water, and some cell biomass which is mostly innocuous protein.
Biodegradation of hydrocarbons and organic waste by bacteria and fungi4 involve the oxidation of the substrate by oxygenases2 such as alkanes, oils, fats, hydrocarbons, cellulose, & sewage waste.
Biodegradation of Sewage, Manure, Agricultural and Food processing Waste.
The biological degradation processes including cellulose wastes in sewage, manure, food processing, and agricultural waste is accomplished by continuous synthetic enzymatic activities of bacterial & fungal4 cells involving the oxidation of the substrate by oxygenases2.
This will simultaneously eliminate the Phosphorus and Nitrates by the same biodegradation process.
Major Metabolic Pathways for Hydrocarbon Biodegradation.
The initial steps in the biodegradation of hydrocarbons and organic waste by bacteria and fungi4 involve the oxidation of the substrate by oxygenases2, for which molecular oxygen O2 is required. (O2 is dissolved air in the liquid solution. Substrate being alkanes, oils, fats, hydrocarbons, cellulose, and sewage waste.)
BioTherm Eliminator enables the combination of oxygen O2 at the molecular level with the substrate, triggering the subsequent conversion of alkanes, oils, fats, hydrocarbons, cellulose and other wastes, to carboxylic acids that are further biodegraded via β-oxidation3 to a harmless reduction of carbon dioxide, water, and cell biomass which is mostly protein and can be safely assimilated into the food chain.
NOTES:
1Biological enzymes are catalysts which act in a narrow operating range of temperature and pH. When these enzymes catalyze a redox reaction they are classified as oxygenases2.
2Oxygenases: Enzymes that oxidize a substrate by transferring the oxygen from molecular oxygen O2 to the substrate, that catalyze reactions in which O2 is introduced into an acceptor molecule.
3β-oxidation is the central metabolic pathway for the utilization of fatty acids from lipids in which two-carbon units are sequentially removed from the molecule with each turn of the cycle, resulting in the formation of acetate which enters the tricarboxylic acid cycle by which alkanes, oils, fats, hydrocarbons, and other wastes are broken down and metabolized so that they can be used as a source of energy in aerobic respiration.
(Aromatic hydrocarbon rings generally are hydroxylated to form diols; the rings are then cleaved with the formation of catechols which are subsequently degraded to intermediates of the tricarboxylic acid cycle.)
4Fungi and bacteria form intermediates with differing stereochemistries. Fungi, like mammalian enzyme systems, form trans-diols, whereas bacteria almost always form cis-diols (many trans-diols are potent carcinogens whereas cis-diols are not biologically active). Since bacteria are the dominant hydrocarbon degraders, the biodegradation of aromatic hydrocarbons results in detoxification and does not produce potential carcinogens.
Benjamin Vroon, Chemist
